The Gulf of Panama (Spanish: Golfo de Panamá) is a gulf of the Pacific Ocean off the southern coast of Panama, where most of eastern Panama's southern shores adjoin it. The Gulf has a maximum width of 250 kilometres (160 mi; 130 nmi), a maximum depth of 220 metres (722 ft) and the size of 2,400 square kilometres (930 sq mi). The Panama Canal connects the Gulf of Panama with the Caribbean Sea, thus linking the Pacific and Atlantic oceans. The Panamanian capital Panama City is the main urban centre on the gulf shore.

The Gulf itself also contains a few minor gulfs, with Panama Bay to the north, Gulf of Parita to the west and Gulf of San Miguel to the east. The gulf has a few islands and on the coast there are a few important ports, like Panama City, La Palma and Chitrè. The Pearl Islands archipelago is a group of over two hundred islands situated to the east in the gulf.

Panama’s largest river, the Tuira, flows south into the Gulf of San Miguel.